Melanin pigment producing marine actinobacterium of Nocardiopsis sp. isolated from marine sediment samples and their anticancer potential against breast cancer cell line

ABSTRACT: INTRODUCTION: Anti-Breast cancer medication is used to treat or prevent cancer. Breast Cancer-prevention drugs are known as anti-breast cancer drugs because they have an anti-cancer activity against cancer-causing stimulation and cancer cells. To test the anticancer activity of Melanin pigment producing marine actinobacterium of the Nocardiopsis species which was isolated from marine sediment samples using an assay known as MTT MATERIALS AND METHODS: The sample was collected from the area Pichavaram mangrove forest area, Tamilnadu. Isolation of actinobacteria was carried out using Kuster agar medium. The colour of the mature sporulating aerial mycelium was recorded in naked eye. The melanoid pigments test was carried out on the media ISP-1 and ISP-7 as recommended by the International Nocardiopsis project. Reverse side pigment production of the isolate was determined on ISP-7 medium. Soluble pigment production of isolate was observed on ISP-7 medium. Spore morphological characters of the strain were studied by inoculating a loop of old cultures into solidified agar containing sterile glass slides. The chemotaxonomic characteristics were also analyzed by the method recommended by the ISP. The production of melanin from potential marine actinobacteria was estimated by the method of sivaperumal et al, 2018. The proliferation of MCF-7 cells was assessed by MTT assay safadi et al, 2003. RESULT: The melanin producing marine Nocardiopsis was isolated and identified. The production of melanin and other parameters were estimated. Further, the melanin showed more than 50% of inhibition after 72 hours by using the MTT assay in the MCF-7 cancer cell line. CONCLUSION: From the current study .We conclude that the melanin from marine Nocardiopsis species showed potential anticancer properties and it might be useful for further research on marine drugs . As we have found that the Nocardiopsis species have over 65% of inhibition after 72 hours at 3µg/ml of extract by using the MCF-7.