Assessing the Capabilities of ChatGPT-4 in Accurately Responding to Frequently Asked Questions Derived from FDA Regulatory Guidelines: A Detailed Evaluation of Accuracy and Relevance in CMC Applications

This study focuses on the design, synthesis and characterization of novel 2,4-thiazolidinedione derivatives with potential anti-convulsant activity. A series of five compounds were synthesized through the reaction of 2,4-thiazolidinedione with various amines and aldehydes, followed by structural modifications aimed at enhancing their pharmacological properties. The synthesized derivatives were characterized to assess their purity. In-silico ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) predictions were performed to evaluate the pharmacokinetic profiles and drug-likeness of the synthesized derivatives. Molecular docking studies were conducted to investigate the binding affinity of selected compounds to AMPA receptors, providing insights into their potential mechanisms of action in seizure modulation. Structure-activity relationship (SAR) studies indicated that specific substitutions at the C5 position of the thiazolidinedione core were critical for enhancing anti-convulsant efficacy. The results indicate that 2,4-thiazolidinedione derivatives may act as promising candidates for further development as anti-convulsant agents, with favorable pharmacokinetic properties and effective binding interactions with relevant targets in the central nervous system.