Workflow for user introduction into microscopy, OMERO and data management at Center for Advanced imaging

At the Center for Advanced Imaging (CAi) at the Heinrich Heine University Düsseldorf, Germany, we have established a workflow to guide users through all aspects of bioimaging. The process begins with an initial consultation with our imaging specialists regarding microscopy techniques for their specific project. Users then receive training in microscope operation, ensuring they can handle the equipment effectively. If needed, our specialists also provide support in image analysis. Next, we introduce users to OMERO, highlighting its features and the advantages of using a bioimage data management system. They are then trained to structure and annotate their data within OMERO according to the Recommended Metadata for Biological Images (REMBI), taking their specific research topics into account. As users prepare for data publication, we assist with data organization and repository uploads. Our goal is to educate researchers in managing bioimage data throughout its entire lifecycle, with a strong emphasis on the FAIR (findable, accessible, interoperable, reusable) principles.Presented at the NFDI4BIOIMAGE All-Hands Meeting (February 2025) in Düsseldorf.