A guide to selecting high-performing antibodies for Cathepsin B (UniProt ID: P07858) for use in western blot

This report presents a guide to selecting high-quality commercial antibodies against Cathepsin B (CTSB) in western blot using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic controls. This study was funded in part by the Michael J. Fox Foundation (MJFF) for Parkinson's disease.

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Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2024-12-10
DOI
https://doi.org/10.5281/zenodo.14365864
Primary Topic
Protease and Inhibitor Mechanisms
Type
article
Field-Weighted Citation Impact
0.00
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article

A guide to selecting high-performing antibodies for Cathepsin B (UniProt ID: P07858) for use in western blot

Zenodo (CERN European Organization for Nuclear Research)
Protease and Inhibitor Mechanisms
article

A guide to selecting high-performing antibodies for Cathepsin B (UniProt ID: P07858) for use in western blot

article en

Abstract

This report presents a guide to selecting high-quality commercial antibodies against Cathepsin B (CTSB) in western blot using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic controls. This study was funded in part by the Michael J. Fox Foundation (MJFF) for Parkinson's disease.

Zenodo (CERN European Organization for Nuclear Research)
Montreal Neurological Institute and Hospital (CA)
Partnerships for the goals
Openalex Percentile: Top 54%
Protease and Inhibitor Mechanisms
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