Analytical Development and HPTLC Method Validation of Mefloquine Hydrochloride in Bulk and Dosage Form

To estimate mefloquine hydrochloride in pharmaceutical dosage form and bulk, a high-performance thin layer chromatographic methodology is easy to use, accurate, fast, and exact has been devised and fully validated. For HPTLC Various proportion of mobile phase were tried by using trial and error methodologies. Mobile phase containing mixture of Acetonitrile: Toluene: Glacial acetic acid (6:3:1) for 10 min and chromatogram run was kept up to 80 mm. good resolution of peaks and distinct peak shapes were observed using this mobile phase composition. Mefloquine hydrochloride showed maximum absorbance at 222 nm using Camag TLC scanner 4 with Vision CAT software. The Rf value of mefloquine hydrochloride was found to be 0.524. The suggested approach can be effectively used to concurrently estimate the drug content of a commercial formulation on a single plate. For regular examination of mefloquine hydrochloride in tablet dosage form and in bulk, it offers a quicker and more economical approach.