α-Amylase Inhibitory Activity and Free Radical Scavenging Activity of Murraya Koeingii Leaves

Murraya koenigii (L.) Spreng, commonly known as curry leaf, has a rich history in traditional medicine, with purported benefits in managing diabetes and other ailments. Murraya koenigii leaves are pinnate with 11 to 21 small, glossy and aromatic leaflets that are bright green in color. The fresh leaves are highly aromatic, with a distinct somewhat citrusy flavour and native to South Asia. This study investigates the dual potential of M. koenigii leaf extracts by evaluating their α-amylase inhibitory activity and free radical scavenging capacity, key factors in hyperglycemia management and combating oxidative stress. Methodology includes the preparation of aqueous and ethanolic extracts of M. koenigii leaves. α-Amylase inhibitory activity was assessed using a standard colorimetric assay with maltose as a positive control. Antioxidant potential was evaluated using FRAP method. The total phenolic and flavonoid content of the extracts were also identified. Aqueous ethanolic extracts of M. koenigii leaves demonstrated significant α-amylase inhibitory activity, with the ethanolic extract exhibiting superior inhibition compared to the aqueous extract, and approaching the activity of the positive control, maltose and α-amylase inhibitory activity was found to be 61.53%. Furthermore, extracts displayed potent free radical scavenging activity in the FRAP method it was about 1.49 reference standard was ascorbic acid indicating significant antioxidant potential. The extract shows positive phytochemicals like phenols and flavonoids. These findings highlight the promising dual therapeutic potential of M. koenigii leaves. The observed α-amylase inhibition suggests a potential role in managing postprandial hyperglycemia, while the strong free radical scavenging activity offers protection against oxidative stress-related complications often associated with diabetes. M. koenigii leaves warrant further investigation as a potential natural therapeutic agent for diabetes management and related oxidative stress.