Preparation And Evaluation of Polyherbal Wound Healing Ointment Containing Annona Squamosa Linn and Phyllanthus Emblica

This study focuses on the formulation and evaluation of a polyherbal wound healing ointment using extracts from Annona squamosa Linn and Phyllanthus emblica. Both plants are traditionally recognized for their antimicrobial, antioxidant, and wound healing properties. The herbal extracts were prepared using the Soxhlet extraction method and subjected to various physicochemical tests including loss on drying, ash value, and water-soluble extractive value. Preliminary phytochemical screening confirmed the presence of flavonoids, phenols, alkaloids, and tannins. The formulated ointment, prepared using standard excipients like wool fat, paraffin, and cetostearyl alcohol, was evaluated for organoleptic properties, pH, spreadability, solubility, washability, and non-irritancy. Antioxidant activity was confirmed using reducing power assays, and antimicrobial activity was assessed through zone of inhibition tests.The results demonstrated that the formulation possesses strong antioxidant and antimicrobial properties, supporting its potential effectiveness as a natural wound healing agent. Herbal remedies were chosen due to their biocompatibility, minimal side effects, cost-effectiveness, and the presence of multiple phytoconstituents that promote synergistic therapeutic action. This polyherbal ointment presents a promising alternative to synthetic wound care products.