Analyzing Calotropis gigantea's Potential as an Anticoagulant Human Fibrinogen: Proteolytic Activity on Latex

Calotropis gigantea, known as “Sweta Arka,” is a perennial herb belonging to the Apocynaceae family, predominantly found in barren regions of India. This plant has been traditionally utilized for various ailments, exhibiting a range of pharmacological activities including analgesic, anti-inflammatory, and anti-diarrheal properties. Recent studies have highlighted its potential in treating cardiovascular diseases, specifically thrombus formation, which is critical in acute coronary syndromes and strokes. The plant’s latex contains hydrolytic enzymes, notably proteases, which may influence hemostasis. This study investigates the proteolytic activity of C. Gigantea latex on human fibrinogen and its implications for coagulation and fibrinolysis. The extraction of active compounds from the leaves was performed using 70% ethanol and methanol, with subsequent analysis revealing the pharmacological properties of these extracts. These findings underscore the potential of C. Gigantea as a therapeutic agent in managing blood coagulation and related disorders.