Method Development and Validation for The Simultaneous Estimation of Pioglitazone and Glimepiride in Bulk and Pharmaceutical Dosage Forms by RP-HPLC

A simple, accurate and precise High Performance Liquid Chromatographic (HPLC) method was developed for the simultaneous estimation of Pioglitazone and Glimepiride in bulk and Pharmaceutical dosage form. Chromatogram was run through Inertsil – C18, ODS column, 150 x 4.6 mm, 5µ. Mobile phase containing Methanol:Acetonitrile taken in the ratio 70:30 was pumped through column at a flow rate of 1 ml/min. Optimized wavelength selected was 289 nm. Retention time of Pioglitazone and Glimepiride was found to be 4.713 min and 6.691 min. The % RSD for Pioglitazone and Glimepiride was 0.042 and 0.057. % Recovery was obtained as 100.16% and 100.29% for Pioglitazone and Glimepiride respectively. Obtained LOD, LOQ values of Pioglitazone and Glimepiride were 0.070, 0.212 and 0.096, 0.293µg/ml respectively. Linearity range was 20 – 80µg/ml for both the drugs. Regression equation of Pioglitazone was y=9884.3x-380.39 and that of Glimepiride is y=56481x-2387.5 Results show that the retention and run time were decreased, so it is evident that the method developed was simple and economical that can be adopted in regular Quality control test in Industries.