In-Silico Design, Synthesis and Antiprolifertive Evaluation of Thiazolidino-Chromone Derivatives

<p><span>Chromones are a group of naturally occurring chemical compound that being existing everywhere in nature, predominantly in plants. The word chromone is derived from the Greak word Chroma,which means colour, which bringing up that most of chromone derivatives can exhibit distinctiveness, in colour.Chromones are the oxygen containing heterocyclic compounds with a benzoannelated γ-Pyrone ring (4H-Chromone-4-one,4H-benzopyran-4-one). Now a days chromone act as a valid scaffold in medicinal chemistry. Our aim was to develop novel thizolidino- chromone derivatives which inhibiting Human Topo (II)α ATPase and provide anti-proliferative activity. In-silico design of novel analogues were carried out using ACD labs ChemSketch 12.0. Molinspiration software was used to analyse ‘Lipinski Rule of Five’ and drug likeness properties. Biological activity was predicted by PASS software. Preliminary docking study was carried out using GLIDE software by SCHRODINGER. Five derivatives which obeyed rule of five and having predicted antitumor activity on Human Topo (II)α ATPase<span>  </span>were synthesized by four<span>  </span>step process. After the completion of reaction in each step, the compounds were isolated, recrystallised by using suitable solvents, purified by TLC and column chromatography. Analogues were characterized by FT-IR, H1NMR, C13NMR and Mass Spectroscopy. The Biological evaluation was done by MTT assay using HCT 116 cancer cell lines. The results were compared with standard anticancer drug 5-Flurouracil.</span><span>The results were compared with standard anticancer drug 5-Flurouracil. The results of present research work showed that novel thizolidino- chromone derivatives have comparable antiproliferative effect with that of standard anticancer drug 5-Flurouracil. This will lead to the development of promising lead compounds for target specific anticancer therapy and encourage further optimization to develop potent antiproliferative agents.</span></p> <p><span> </span></p>