Comparative Study Of Vitamin B12, Iron, Zinc And Magnesium In Herbs Of Maharashtra

Medicinal plants are the spine of traditional medicine. These therapeutic plants are rich sources of ingredients which can be utilized as a part of medication, synthesis and development. Here a comparative study has been performed for vitamin B12,zinc, iron and magnesium in the herbs of Maharashtra namely Black Mustard, Chick pea and Beet root for treating various vitamin B12 deficiency disorders. The vitamin B12 deficiency is mostly caused by anaemia, impaired intestinal absorption, malnutrition etc. In this comparative study an analytical method called HPTLC is used for quantification of vitamin B12 and Atomic Absorption Spectroscopy was performed for quantification of iron, zinc and magnesium. The extract of each plant is Phytochemically evaluated with various Phytochemical screening for Alkaloids, Glycosides, Tannins, Carbohydrates etc. The High Performance Thin layer Chromatography is performed on the alcoholic extract of these plant materials. The mobile phase is used for HPTLC is Methanol: Ammonia: 2-Propanol: Formic acid: Water (1:1:7:0.5:2) and stationary phase used is Pre-coated silica G gel 60F 254. By the study of High Performance Thin Layer Chromatography we have determined the amount of vitamin B12 present in each alcoholic plant extract. These herbal plants can be used for treatment of vitamin B12 deficiency disorders. In atomic absorption spectroscopy all the sample solutions were run by hallow cathode lamps.