Phytochemical screening and evaluation of Antimicrobial and Antioxidant activity of Cannabis sativa leaves extract

Objectives: The study focuses on extracting and analyzing bioactive compounds from theplant's leaves, particularly cannabinoids and flavonoids, known for their antibacterial andantioxidant properties. By optimizing extraction methods and assessing these compounds'efficacy, the research aims to enhance the understanding of Cannabis sativa therapeuticpotential in alternative medicine.Methods: This study extracts bioactive compounds from Cannabis sativa using Soxhletextraction with methanol and petroleum ether. The extracts are screened for phytochemicalsand tested for antibacterial activity against Gram-positive and Gram-negative bacteria, as wellas antioxidant activity using the DPPH assay, to assess its potential medicinal properties.Result: Cannabis sativa leaves were extracted with methanol (20.76% yield) and petroleumether (14.1% yield). The methanol extract showed higher antioxidant activity (IC50 = 98.1µg/ml) compared to petroleum ether (IC50 = 106.43 µg/ml), with ascorbic acid showing thehighest activity (IC50 = 16.2 µg/ml). The methanol extract also exhibited strong antibacterialeffects, particularly against E. coli and Pseudomonas, with inhibition zones up to 20 mm.Phytochemical analysis revealed alkaloids, terpenoids, tannins, and glycosides in both extracts,with methanol also containing resins, suggesting it is more effective for extracting bioactivecompounds from Cannabis sativa.Conclusion: Cannabis sativa leaves exhibit notable antioxidant and antibacterial properties.The methanolic extract, with the highest antioxidant activity (IC50 = 98.1 µg/ml) and effectiveantibacterial action.