First records of protosteloid amoebae isolated from leaf litter in Singapore

Nine new records of protosteloid amoebae are reported from 88 samples of leaf litter collected between January and June 2024 from waste-woodland and native-dominated secondary tropical rainforests in Singapore. They are Acanthamoeba pyriformis, Cavostelium apophysatum, Ceratiomyxella tahitiensis/Nematostelium gracile, Protostelium mycophaga, Schizoplasmodiopsis amoeboidea, S. pseudoendospora, S. vulgaris, Tychosporium acutostipes, and Vanella fimicola. This is the first survey conducted on the occurrence of protosteloid amoebae in Singapore and the second in Southeast Asia.