Development of a Probiotic-Based Health Supplement: Screening, Characterization, and Stability Assessment

Probiotic-based health supplements have gained considerable attention due to their potential benefits in improving gut health, immunity, and overall well-being. This study focuses on the screening, characterization, and formulation of a probiotic health supplement, emphasizing stability assessment to ensure long-term efficacy. Lactic acid bacteria (LAB) were isolated from dairy samples collected from various sources and characterized using biochemical and molecular techniques. Their ability to survive under different pH conditions, bile salt concentrations, NaCl levels, and cholesterol assimilation was evaluated. Acid tolerance and adhesion assays were performed to determine their probiotic potential. A probiotic formulation for skin health was developed incorporating a probiotic blend, hyaluronic acid, grape seed extract, vitamins, and inulin. The formulation was subjected to stability testing at controlled environmental conditions to assess microbial viability and physicochemical properties. This research provides a comprehensive approach to developing a stable probiotic-based health supplement with enhanced functional benefits.