In Vitro Study of Antibacterial Activity of Flower Extract of Cassia auriculata on Human Pathogens

Abstract Plants are one of the most important and a rich source of medicine as it produces different types of bioactive compounds and are used as medicine from ancient period. Due to the indiscriminate use of antibiotics has led to an increase in antibiotic resistance among microorganisms. Bacteria become resistant to antibiotic by different ways. R-plasmid often contains genes for resistance to different antibiotics. Patient suffering from antibiotic resistant strain fail to respond antibiotic treatment. So there is a continuous demand of new drug. In the present study antibacterial activity of flower of Cassia auriculata was studied against Escherichia coli NCIM2064, Staphylococcus aureus NCIM2079, Proteus mirabilis NCIM4175 , Pseudomonas aeruginosa NCIM 2036, Salmonella typhi and Shigella dysentery .The antibacterial activity of water and solvent (ethanol and methanol) extract of plant was studied by agar - well diffusion method. Cassia auriculata showed maximum antibacterial activity against Staphylococcus aureus NCIM2079, Salmonella typhi, Escherichia coli NCIM2064, Shigella dysentery and least activity against Pseudomonas aeruginosa NCIM 2036, Proteus mirabilis NCIM4175. The flower extract of Cassia auriculata could be used as potential source of herbal medicine against human pathogenic bacteria.