Citrus aurantium bark, seeds, and leaves were used to synthesize and characterize silver nanoparticle and their antimicrobial activity was evaluated

The green synthesis of silver nanoparticles has been proposed as an eco-friendly and cost-effective substitute for chemical and physical methods. The aim of this study was to synthesize and characterize silver nanoparticles using the peel extract of Citrus aurantium Bark, Leaf and Seed, and to determine the possible phytochemical constituents’ presence in the plant extracts that might be responsible for the synthesis. Citrus aurantium Bark, Leaf and Seed extraction was followed by phytochemical studies of secondary metabolites, FTIR analysis confirmation of functional groups analysis. Silver nanoparticles were synthesized through bio-reduction of silver ions to silver nanoparticles using Citrus aurantium Bark, Leaf and Seed and characterized using UV-Vis spectroscopy (Bark, Leaf and Seed), SEM (Bark and Leaf), XRD (Bark, Leaf and Seed) and FTIR (Bark Leaf and Seed). The FTIR analysis of the extract revealed the presence of functional groups like hydroxyl, carboxyl, carbonyl, amine, and phenyl with similar functional groups. The synthesized silver nanoparticle (AgNP) has displayed the characteristics of a UV-Vis spectroscopy band peak from 400–420 nm. The XRD analysis also confirmed that the nanoparticles synthesized are crystalline in nature. Based on the findings of this study, it is understood that the variety of natural compounds that are present in plant extracts of Citrus aurantium Bark, Leaf and Seed can act as both reducing and stabilizing agents for the synthesis of silver nanoparticles. It is, therefore, concluded that Citrus aurantium Bark, Leaf and Seed extract can be potentially used for the large production of silver nanoparticles for several applications. published by the Journal of Biodiversity and Environmental Sciences (JBES)