Intestinal Mucosal Damage and Intestinal Permeability In Non Infectious and Infectious Diarrheic Calves In Relation to Diamine Oxidase Activity

This field study was designed as a cohort (prospective) and involved 71 calves within the borders of Aydın Province. Each calf in the study was from one of three different farms (Farm A with 12 non-infectious diarrheic, 15 infectious diarrheic, Farm B with 13 non-infectious diarrheic, 19 infectious diarrheic, and Farm C as the healthy control group n=12). Serum samples were separated into Eppendorf tubes after centrifugation and stored in a -80 °C freezer until analysis. The competitive enzyme immunoassay technique was applied using the Bovine Diamine Oxidase ELISA test. Diamine Oxidase (ng/mL) measurements in infectious, non-infectious, and healthy calves were determined to be 6.52 ± 2.85, 7.16 ± 3.40, and 17.05 ± 2.63 (p< 0.001), respectively, in terms of mean ± standard error. The data obtained suggest that determining circulating diamine oxidase enzyme levels under field conditions can support diagnosis as a biological marker of intestinal permeability, and more prominently, diamine oxidase activity may decrease in both non-infectious and infectious diarrheic calves in relation to small intestinal mucosal damage. This could represent a step closer to the diagnosis of leaky gut in calves.