Studies on the Evaluation of Flavonoids (Morin, Naringin, Quercetin & Rutin) in Blackberry Leaves By HPLC

Flavonoids are polyphenolic compounds known for their antioxidant properties and various health benefits. The current study focused on the evaluation and quantification of flavonoids Morin, Naringin, Quercetin, and Rutin in blackberry (Rubus fruticosus) leaves. High-Performance Liquid Chromatography (HPLC) is employed for the qualitative analysis of these compounds using a reverse-phase column with 0.1% phosphoric acid and acetonitrile in a 25:75 ratio. The standard retention times for Quercetin, Morin, Naringin, and Rutin were 2.513 min, 3.887 min, 7.130 min, and 4.780 min, respectively. In blackberry leaf extracts, Quercetin, Morin, and Naringin are identified at retention times of 2.567 min, 3.640 min, and 5.673 min, respectively, these results confirm the presence of key flavonoids with potential health benefits in blackberry leaves.