Camporesiomyces coffeae M. Y. Han & Tibpromma 2025, sp. nov.

Camporesiomyces coffeae M. Y. Han & Tibpromma sp. nov. Fig. 3 Etymology. The species epithet “ coffeae ” refers to the host genus Coffea. Holotype. GMB-W 1181 Description. Saprobic on dead branches of Coffea arabica. Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies on natural substrate superficial, solitary or clusters, hairy, subhyaline at apex, bunch of flowers-like, shiny. Mycelium exposed on surface of substrate, except on the roots. Conidiophores 43–97 × 2.8–4.5 μm (x ̄ = 68 × 3.7 μm, n = 30) macronematous, mononematous, either solitary or forming small clusters, smooth or occasionally verruculose, cylindrical, dark brown, slightly flexuous, simple, unbranched, with longitudinal striations in the upper part, 2–9 - septate, sometimes slightly constricted at the septa. Conidiogenous cells 8–21 × 2.3–4.6 μm (x ̄ = 15.4 × 3.4 μm, n = 30), polyblastic, terminal, cylindrical, with longitudinal striations, with several conspicuous denticles at apex, brown. Conidia 20–50 × 3.3–6.5 μm (x ̄ = 26.7 × 4.5 μm, n = 30), solitary, acrogenous, guttules, cylindrical, fusiform, 3–7 - septate, subhyaline or hyaline, obtuse or conical at both ends. Culture characteristics. Conidia germinating on PDA within 24 h at 28 ° C, colony on PDA reaching 2 cm diam. after two weeks, circular or irregular, umbonate, with entire margin dark grey to black, irregular, the reverse side displays predominantly black colonies with brown peripheral edges. Material examined. China • Yunnan Province, Pu’er City, Simao District (22°35'53"N, 100°59'17"E, 1186.4 m), on dead branches of Coffea arabica, 8 August 2024, M. Y. Han & Tibpromma, (YYT 15 = GMB-W 1181, holotype), ex-type GMBCC 1130; other living culture GMBCC 1131. GenBank number. GMBCC 1130 = ITS: PQ 763358 LSU: PQ 842545 tef 1 - α: PV 388890, rpb 2: PV 388896 and GMBCC 1131 = ITS: PQ 763359 LSU: PQ 842546 tef 1 - α: PV 388891, rpb 2: PV 388897. Notes. Based on multi-gene phylogenetic analysis, Camporesiomyces coffeae [GMBCC 1130 (ex-type) and GMBCC 1131] is a distinct species, sister to C. puerensis [GMBCC 1113 (ex-type) and GMBCC 1114] with 98 % ML / 1.00 PP support (Fig. 1). Comparative genomic analysis demonstrated that the sequence similarity between C. coffeae and C. puerensis was 93.00 % in the ITS locus, 98.33 % in the LSU locus, 93.34 % in the tef 1 - α locus, and 89.03 % in the rpb 2 locus (Table. 3). Morphologically, C. coffeae can be distinguished from C. puerensis by the dimensions and septation of conidiophores and conidia, in that the conidia and conidiophores of C. coffeae are smaller than C. puerensis (20–50 × 3.3–6.5 μm vs. 21.7–83 × 4–9.4 μm) and (43–97 × 2.8–4.5 μm vs. 52–176.5 × 2.8–5.6 μm), respectively (Figs 3, 4). In addition, the conidia of C. coffeae are subhyaline or hyaline, whereas those of C. puerensis are hyaline at their ends but yellow in the middle. Moreover, the conidiophores of C. coffeae are distinguished by longitudinal striations in their apical sections, a feature that is absent in C. puerensis. Camporesiomyces coffeae is similar to C. puerensis in having polyblastic, terminal, conidiogenous cells, which are brown (Figs 3, 4). Based on the differences between the two species, we describe C. coffeae as a new species.