Various Methods of Phytoconstituent Estimation

This review explores the diverse analytical methods used to assess phytoconstituents in medicinal plants, focusing on their unique characteristics and therapeutic potential. Key techniques such as UV-VIS spectroscopy, Paper, thin-layer, high-performance liquid, and gas chromatography are examples of chromatography, and various spectroscopic methods (FTIR and mass spectrometry) are highlighted for their roles in the detection, isolation, and measurement of bioactive substances. The efficacy of these methods in evaluating antioxidant properties and quality control in phytopharmaceuticals is underscored, with a particular emphasis on developing a validated UV-visible spectrophotometric approach for routine laboratory analysis. This work aims to improve the accuracy and efficiency of phytochemical analysis, facilitating better understanding and utilization of medicinal plants in healthcare.