Green Synthesis of Mg Nanoparticles Using Ocimum Basilicum (ObE-MgNPs): Characterization, Acute Toxicity Evaluation and Hepato-Protective against Side Effect of Doxorubicin Chemotherapy in Rats

This investigation was aimed to evaluation of Doxorubicine a chemotherapy substance induced hepatotoxicity and the preventive effect of ObE-MgNPs in rats. Standard procedures were used to extract bioactive molecules and test qualitative phytochemical substances. Furthermore, visual observation, FT-IR spectroscopy, and scanning electron microscopes (SEM), techniques were used to verify the green synthesis of ObE-MgNPs. For in-vivo rats study, 15 female albino Wistar rats were divided into 3 groups (n = 5); control group, doxorubicin treated rats group (DOX) and rats co-treated with doxorubicin and MgNPs group (DOX + MgNPs). Various hematological, enzymatic, and oxidative stress markers were estimated. The FT-IR spectroscopy analysis confirmed the synthesis of SeNPs and scanning electron microscope (SEM) analysis revealed the spherule-like structure of MgNPs with average size around 78.5 nm. Phytochemical results appeared the richness of Ocimum basilicum L. extract by various phenolic compounds. In in-vivo study results show that doxorubicin induce an alteration in hematological parameters and enzymatic activities compared to control group. In addition, doxorubicin treated rats induced oxidative stress in liver cells compared to control rats. Co-treatment of doxorubicin with MgNPs were partially reversed all of previous parameters. This study indicated that the antioxidant property of Magnesium nanoparticles allowed using them to protect organs from the side effects of doxorubicin or from the destructive effects of various diseases.