Development And Validation Of RP- HPLC Method For Estimation Of Lercanidipine HCL In Bulk Drug And Dosage Form

A simple, precise, reliable, rapid and reproducible reversed-phase high performance liquid chromatography method was developed and validated for the simultaneous estimation of Atenolol (ATL) and Lercanidipine Hydrochloride (LER) present in tablet dosage forms. Chromatographic separation achieved isocratically on Luna C18 column (5 µm, 150mm x 4.60mm) and ACN/phosphate buffer (60:40, v/v, pH 3.6) as mobile phase, at a flow rate of 0.5ml/min. Detection was carried out at 235 nm. An effective chromatographic separation was achieved using waters symmetry C18 column of dimensions 150x4.6 mm, 3.5 μm, as a stationary phase. 0.1 percent ortho phosphoric acid and acetonitrile in 50:50 v/v was used as a mobile process with a rate of flow 1 ml/min and UV detection was carried out at 230 nm, respectively. Isocratic chromatography at ambient temperature was performed.