Identification and Isolation of E. Coli Bacteria from Ro Water in the Center of Karbala and its Surrounding Areas

The aim of this research was to find out if the water (Ro) in the majority of Iraqi houses contains E. coli bacteria because of the prevalence of diarrhea and other illnesses among the populace. The goal was accomplished by. The study samples were collected from R. O water filling stations in Karbala, including 40 samples from Karbala Center, 20 samples from Tuwairij District, 20 samples from Al-Hussainiya District, and 20 samples from Ain Al-Tamr District for the purpose of investigating the E. coli bacteria contaminating these waters, where microscopic and cultural examinations and biochemical tests were used. The results of these tests showed that 3 samples of R.O water out of 100 samples, with an isolation rate of 4.5%, gave positive results through their growth on selective and differential media and their fermentation of lactose sugar. On the other hand, the research results showed that the highest percentage of bacteria isolation was from R.O water filling stations in Ain Al-Tamr district with a percentage of 2.5) 2.2%) and the lowest percentage was from R.O water filling stations in Al-Hussainiya district with an isolation percentage of 1.7) 1.5%), while R.O water filling stations in the center of the governorate of Karbala and Twerij district did not record the least contamination with coliform bacteria (0.5 %). Through the research, the results showed that R. O water in some areas is contaminated with E. coli bacteria, which may be the source of human waste, which indicates the inefficiency of these stations.