Bulk TCR-seq from paired EAT, Atrial appendage and blood samples

Bulk TCR alpha and beta sequencing was performed from paired blood AA and EAT samples from 5 AF patients. In addition, TCR sequencing was performed using additional EAT from 5 SR patients. cDNA was extracted from tissues and whole blood. A quantitative experimental and computational TCR sequencing pipeline was employed as previously described in Peacock et al. The pipeline introduces unique molecular identifiers (UMI) attached to individual cDNA TCR molecule allowing correction for PCR and sequencing errors. TCR identification, error correction and CDR3 extraction was performed following a suite of tools available at github.com/innate2adaptive/Decombinator as detailed in Peacock et al.