Study Of Antibacterial Activity & Phytochemical Test Of Thuja Occidentalis Root L. (Cupressaceae)

Thuja occidentalis (commonly morpankhi) is a genus of coniferous tree in the cupressaceae (cypress family). In this study, Thuja root were collected from local area sarojini nagar, and dried under tray dryer for 10 days. They were then grinded using mechanical grinder.and extracted with a Soxhlet extractor in two solvent systems, i.e. (F1) ethyl acetate: acetone: ethanol (40:30:30) and (F2) methanol: distilled water (70:30). This study conferred the screening of phytochemical constituents. This study examined the antibacterial activity of Thuja roots extract on gram positive (Staphylococcus aureus and enterococcus faccalis and bacillus subtilis.) and gram-negative bacteria (E. coli ). Root extract was obtained by soxhlet extraction technique using mixture of Ethyl acetate, acetone, ethanol ( non aqueous Extract) ratio 40:30:30 and methanol , water ( aqueous extract) ratio 70:30 as the solvent. The antibacterial activity of Thuja occidentalis was tested Standardization of crude drugs extracted from plants plays a vital role in identifying the quality and purity of medications. Current research analysis reveals standardization of crude drugs that includes moisture content, acid-insoluble and water-soluble, and different soluble extraction values were estimated. The highest extractive values were recorded for the water-soluble extract of crude drugs, bioactive substances present in medicinal plants that can lead to drug discovery and development. The present study deals with the phytochemical components of the medicinal plant Thuja occidentalis from the Cupressaceae family in order to relate their presence to the bioactivity of the plants. Current research highlights that methanolic extracts of Thuja occidentalis had the highest number of phytochemicals compared to other solvent extracts. Therefore, methanolic extracts of Thuja occidentalis have the most significant potential to treat various human diseases and have profound medical utility.