A Review On HPLC Analytical Method And Validation For Determination Of Sitagliptin Phosphate In Bulk And Different Dosage Form

A new simple, rapid, specific, accurate, precise and novel Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the estimation of Sitagliptin Phosphate in the pharmaceutical dosage form. The chromatographic separation for Sitagliptin was achieved with mobile phase containing methanol, Thermoscientific C18 column, (250x4.6 particle size of 5µ) at room temperature and UV detection at 267 nm. The compounds were eluted in the isocratic mode at a flow rate of 1ml/min. The retention time of Sitagliptin was 2.37min. The above method was validated in terms of linearity, accuracy, precision, LOD, LOQ , interday, intraday , selectivity , range , stability and robustness in accordance with ICH guidelines.