Formulation And Development And In Vitro Evaluation Of Ocimum Sanctum Leave Microemulgel For Antioxidant Activity

This study aimed to formulate and evaluate Microemulgel loaded with Ocimum sanctum leaf extract for antioxidant activity. Leaves were extracted using methanol, ethanol, and water, with ethanol providing the highest yield. The phytochemical analysis demonstrated the presence of bioactive compounds like alkaloids, flavonoids, terpenoids, and steroids in the extracts. Three formulations of Microemulgel were developed using different polymers - Carbopol 934, Carbopol 940, and HPMC. The formulated Microemulgel exhibited desirable properties including slightly acidic pH, high viscosity, and good spreadability, aligning them well for topical administration. In vitro drug release studies demonstrated a sustained release of active compounds over an 8-hour period, indicating the potential for long-lasting therapeutic effect. All formulations had a nanometric particle size and a stable zeta potential, confirming their stability and effective drug delivery capability. The antioxidant activity, assessed via the DPPH radical scavenging method, revealed significant potential, particularly in the Carbopol 934 formulation. These findings suggest that Ocimum sanctum leaf extract microemulgels could be promising candidates for antioxidant skincare applications. However, in vivo studies and clinical trials are needed to further confirm their safety and efficacy.