Exploration of adulterants and total minerals in LAE, KAE, SAE and MAE

The aim of this work is to explore the adulterants and total minerals in LAE, KAE, SAE and MAE. HPLC and GC-MS chromatogram conforms the adulterants were present in the LAE, KAE, SAE and MAE as it elute 3, 4, 3, 3 peaks in HPLC and 10, 10, 7, 6 peaks in GC-MS respectively. In addition, LAE, KAE, SAE and MAE also withhold several minerals and heavy metals such as zinc, manganese, iron, aluminium, chromium, copper, nickel and strontium. Moreover, LAE, KAE, SAE and MAE possess non-toxic property as it was unable to cleave packed RBC in in-vitro study at the concentration of 200µg. Astonishingly; LAE, KAE, SAE and MAE exhibits anti-microbial property as it produce zone of inhibition in both gram negative bacteria E. coli and gram positive bacteria S. aureus. The obtained anti-bacterial results are demonstrated in Minimum Inhibitory Concentration (MIC) values for LAE, KAE, SAE and MAE.