Stage-specificity profiling.

Stage-specific dose response assays for compounds <b>(A) 22</b>, <b>(B) 31</b>, and <b>(C) 33</b>. Assays were performed on tightly synchronised cultures, with compound applied for 10 h at the ring (6–16 h post-invasion), trophozoite (18–28 h post-invasion) and schizont (30–40 h post-invasion) stages. After drug washout, cultures were incubated for a further 62 h. For comparison, assays were also performed with constant drug pressure applied for the full 72 h. The bar graph (<i>left</i>) represents mean IC₅₀ values for three replicates, the survival graphs (<i>right</i>) display the dose response curves from three independent experiments. Error bars indicate the standard error of the mean based on these three independent repeats. (PDF)