Detection of the native <i>LINE-1</i> sequences after bisulfite conversion.

<p>(<b>A</b>) The PCR product was successfully amplified with the primers specific to the native <i>LINE-1</i> from bisulfite-treated DNA with 5ng, 50ng, and 500 ng, on DNA samples of breast (1), colon (2) and lung tumour (3) tissues. However, no native <i>LINE-1</i> product was detected when the DNA input was 0.5 ng. (—): Negative control without DNA template. (<b>B</b>). Direct sequencing of PCR products amplified from bisulfite-treated DNA with an input amount of 0.5 ng using the MIP primers and an input amount of 50 ng using the primers specific to the native <i>LINE-1</i> sequences. Almost non-CpG cytosines remained in DNA input of 50 ng. Rare conversions of non-CpG cytosines were underlined.</p> <p>(PDF)</p>