Effects of CPS deacetylation on virulence in a pneumonia model using both female and male mice.

<p>(A-D) CPS deacetylation (ΔwcsU) reduces virulence in a pneumonia model using female mice. (A) Survival rates of mice. Bacterial burdens in lungs (B), livers (C), and spleens (D) were indicated, respectively. (E-H) CPS deacetylation (ΔwcsU) reduces virulence in a pneumonia model using male mice. (E) Survival rates of mice. Bacterial burdens in lungs (F), livers (G), and spleens (H) were indicated respectively. Mouse survival rates were monitored 7 days post intranasal infection with 2,000 CFU of various ATCC43816 strains. Bacterial burdens in organs were determined 48 h post intranasal inoculation. Each dot represents one mouse. The Log-rank (Mantel-Cox) test was performed to compare the survival rates. Unpaired t-test was performed to determine the statistical significance among bacterial burdens in organs.</p> <p>(PDF)</p>