Showing how cell area changes depend on antibiotic concentration for <i>E.coli</i>, <i>S.aureus</i> and <i>P.aeruginosa</i>.

The plot for <i>E.coli</i> is also shown in the main text but repeated here for completeness. For each species, curves are shown for all the tested antibiotics after 2.5 hours of incubation with the antibiotics. The antibiotic concentrations are normalised to growth MIC, and the cell area is normalised so that the no-antibiotic area is 0 and the maximum change in cell area is 1 (which for vancomycin entails inverting the area). The MOR₅₀ threshold is shown as a horizontal line at 50% change, and the cell MIC is where the area curve first crosses this threshold. All the antibiotics produce a morphology change around the growth MIC. The degree of change varies strongly between antibiotics, which is here made apparent through the noise present in the area signal for antibiotics that induces a small change in morphology. The antibiotics are categorised by action mechanisms as inhibiting cell wall synthesis, protein synthesis, and nucleic acid synthesis, in addition to a negative control where no antibiotic was used. The plot for <i>E.coli</i> with normalized cell area is also presented in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012924#ppat.1012924.g007" target="_blank">Fig 7</a> and is included here for completeness. (PDF)