Results of the sequence analysis (ITS region and D1/D2 domain) of closely related yeast species to <i>Glaciozyma martinii</i> 186 using BlastN. Results with the highest percentage of identity.

Among the selected species with the highest percentage of identity to the tested sequence (ITS region and D1/D2 domain) were: <i>Glaciozyma martinii</i>, <i>Glaciozyma antarctica</i>, and <i>Glaciozyma watsonii</i>. <i>Glaciozyma martinii</i> exhibited the highest percentage of identity (over 99%), with sequence coverage for the selected phylogenetic marker close to 100% and minimal gaps. <i>Glaciozyma antarctica</i> displayed a lower percentage of identity at approximately 94% and a higher content of gaps (2%–3%) with coverage close to 100%. <i>Glaciozyma watsonii</i> showed a similar situation to <i>Glaciozyma antarctic</i>a. <i>Phenoliferia himalayensis</i> exhibited a much lower identity at 89.39% with nearly 100% coverage. The sequence identity of <i>Rhodotorula svalbardensis</i> was at 82.89% with a sequence coverage value of 85%. <i>Zymoxenogloea eriophori</i> demonstrated the lowest identity with 100% sequence coverage. From the listed sequences, those suitable for phylogenetic analysis using the MEGA11 software were selected. (PDF)