Confirmation of <i>far8</i>Δ deletion mutants.

<p>(A) PCR genotyping of the 5’ and 3’ junctions for representative <i>far8</i>Δ mutants in the H99α background. WT (H99α) served as a negative control. (B) Snapshot of DNA read coverage at the <i>FAR8</i> locus from Illumina whole genome sequencing in a <i>far8</i>Δ mutant strain. The coding sequence is depicted in yellow and individual DNA reads are shown below in black. The <i>FAR8</i> gene was deleted from the middle of exon 1 to the middle of the last exon (exon 7), as verified by the absence of reads. The read coverage for the indicated chromosomal coordinates is shown in blue.</p> <p>(DOCX)</p>