Sorting of CD8<sup>+</sup> T cell subsets for WGBS.

<p>PBMCs from healthy CMV-seropositive donors were pre-enriched for CD8<sup>+</sup> T cells and stimulated with CMVpp65 overlapping peptide pool to detect IFN-γ-secreting T(CMV) cells using IFN-γ Secretion Assay/Detection Kit. Representative flow cytometric plots show the gating strategy for sorting of T<sub>N</sub> (CD3<sup>+</sup>CD4<sup>−</sup>CD8<sup>+</sup>CCR7<sup>+</sup>CD28<sup>int</sup>KLRG1<sup>−</sup>CX<sub>3</sub>CR1<sup>−</sup>CD45RA<sup>high</sup>) (orange gate), T<sub>mem</sub> (CD3<sup>+</sup>CD4<sup>−</sup>CD8<sup>+</sup>CCR7<sup>−</sup>CD28<sup>high</sup>CD27<sup>+</sup>CD45RA<sup>−</sup>) (light blue gate), and T(CMV) cells (CD3<sup>+</sup>CD4<sup>−</sup>CD8<sup>+</sup>IFN-γ<sup>+</sup>) (red gate) from 5 donors.</p> <p>(PDF)</p>