Generation of fluorescent <i>mac1</i>Δ- and <i>mac1</i>Δ<i>ctr3</i><sup>OE</sup><i>fre9</i><sup>OE</sup> strains expressing 3X<i>mClover3</i>.

<p><b>(A)</b> Physical map of the <i>Fo-mClover3</i> expression cassette used to transform <i>mac1</i>Δ or <i>mac1</i>Δ<i>ctr3</i><sup>OE</sup><i>fre9</i><sup>OE</sup> #4 strains. Relative positions of the primers used for diagnostic PCR are indicated. <b>(B, C)</b> Agarose gel electrophoresis of PCR products obtained using the primer pair Gpda4 and 3XFLAGrev with genomic DNA extracted from the indicated phleomycin-resistant transformants or <i>mac1</i>Δ or <i>mac1</i>Δ<i>ctr3</i><sup>OE</sup><i>fre9</i><sup>OE</sup> #4 as negative controls. M, molecular size markers.</p> <p>(PDF)</p>