Impact of high-sugar diets on survival of infection with Gram-positive bacteria.

<p>(A) There was no effect of diet on survivorship after infection with <i>Enterococcus faecalis</i> at an inoculation dose of ~1000 colony forming units (CFU) per host. (B) Flies fed the 16% sucrose diet have significantly higher mortality than flies fed 2% sucrose with a higher infection dose (~3000 CFU/fly) of <i>E</i>. <i>faecalis</i> (p = 0.01; Cox Mixed Effects Model), while there was still no effect of diet on survivorship with <i>E</i>. <i>faecalis</i> in a replicate experiment at an inoculum of ~1000 CFUs (p = 0.14). Dashed lines indicate a lower infection dose (~1000 CFU/fly) and solid lines indicate a higher inoculation dose (~3000 CFU/fly). (C) Flies fed the 24% sucrose diet have the highest mortality after <i>Lactococcus lactis</i> infection compared to all other diets at an inoculation dose of ~1000 CFU/fly (p<0.001, Cox Mixed Effects Model). Flies fed 16% sucrose died significantly faster than flies fed 0% sucrose diets (p = 0.047; Cox Mixed Effects Model), but there were no other significant pairwise differences in survivorship among flies provided the 16% sucrose diet or lower. Letters denote significant pairwise differences between diets (p<0.05). (D) Flies fed the 16% sucrose diet have significantly higher mortality than flies fed 2% sucrose after <i>L</i>. <i>lactis</i> infection with ~1000 CFU/fly (p< 0.001; Cox Mixed Effects Model) or ~3000 CFU/fly infection doses (p < 0.001; Cox Mixed Effects Model). Dashed lines indicate a lower infection dose (~1000 CFU/fly) and solid lines indicate a higher inoculation dose (~3000 CFU/fly).</p> <p>(DOCX)</p>