Minimum inoculum for resistance (MIR).

Three independent cultures of either the parental Dd2 or isogenic Dd2-Polδ mutator lines were exposed to compound <b>22</b> or GNF179. Parasite inoculum ranged from 10⁴ to 10⁸ parasites per culture. Drug pressure was initiated at 3×IC₅₀ for both compound <b>22</b> (90 nM) and GNF179 (10.5 nM). For compound <b>22</b>, the selection pressure was raised to 4×IC₅₀ (120 nM) at day 6 and 5×IC₅₀ (150 nM) at day 9 in order to fully clear the culture. Red squares indicate absence of parasites after 45 days (GNF179) or 54 days (compound <b>22</b>), green squares indicate recrudescence, with the day of recrudescence representing the day when parasitemia crossed 1%. (PDF)