Pretreatment with linsitinib reduces IGF-1-increased the proportion of S-phase cells in OFs from TAO patients.

<p>Representative histogram of the gated cells in the G0/G1, S, and G2/M phases of linsitinib-pretreated or/and IGF-1-treated OFs from TAO patients. OFs from TAO patients were plated at a concentration of 2 x 105 cells/well in a 6-well plate. After 24 h, the cells were pre-treated with linsitinib at the indicated concentrations for 2 h, followed by treatment with 50 ng/ml IGF-1 for 24 h. After 24 h, cells were collected and stained with propidium iodide (PI) and their DNA contents were analyzed by flow cytometry.</p> <p>(PDF)</p>