Influence of RNase III on <i>lcrF</i> transcription.

<p>(<b>A</b>) Plasmid pMV53 encoding the <i>yscW-phoA</i> transcriptional fusion and (<b>B</b>) plasmid pKB35 encoding the <i>yscW-lacZ</i> transcriptional fusion were transformed into <i>Y</i>. <i>pseudotuberculosis</i> strains YPIII (wt) pAKH85 (pV, empty vector), YP356 (Δ<i>rnc</i>) pAKH85 (pV, empty vector) and the complementing strains YPIII (wt) pIVO20 (p<i>rnc</i><sup>+</sup>) and YP356 pIVO20 (p<i>rnc</i><sup>+</sup>). The transformants were grown at 37°C and alkaline phosphatase or beta-galactosidase activity was determined, respectively. Data represent the mean ± SD from three independent biological replicates. Significant differences were determined using Student’s t-test and indicated by asterisks (*P <0.05).</p> <p>(PDF)</p>