S10 Fig -

<p><b>(A)</b> Fluorescence microscopy of the cell line overexpressing V5-tagged GiMlf. Top panel–Presence of large vesicular structures containing GiMlf in the vicinity of mitosomes. Stained with anti-V5 antibody (green) and anti-GL50803_9296 antibody (mitosomal marker, magenta). Nucleic DNA was stained with DAPI (blue). DIC image of corresponding cell is shown in corner of the merged image. Scale bar: 2 μm. Bottom panel–Expansion microscopy of the cell line overexpressing GiMlf-V5. Stained with anti-V5 antibody (green) and anti-PDI2 antibody (ER marker, magenta). Scale bar: 10 μm. <b>(B)</b> Transmission electron microscope image of the cell line overexpressing GiMlf-V5 and at low and high magnification, stained with an anti-V5 antibody. The blue arrow points to a peripheral vacuole. Scale bars: 200 nm.</p> <p>(DOCX)</p>