Hypusination of yeast eIF5A by <i>H</i>. <i>sapiens</i> and <i>P</i>. <i>vivax</i> DHS-complemented strains.

<p>(A) Western blot detection of hypusinated eIF5A and total eIF5A from the <i>S</i>. <i>cerevisiae</i> strains wt, <i>dys1Δ</i>::<i>HsDHS</i> and <i>dys1Δ</i>::<i>PvDHS</i> (SFS01, SFS04 and SFS05, <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0012690#pntd.0012690.s014" target="_blank">S2 Table</a>). An antibody specific for <i>S</i>. <i>cerevisiae</i> DHS (ScDHS) was used to show the deletion of the endogenous gene in the strains replaced by HsDHS and PvDHS. (B) Inhibition of growth of <i>P</i>. <i>vivax</i> DHS-complemented by different methionine concentrations. (C) Western blot detection of hypusination levels after 10 h of methionine treatment (concentrations indicated in the figure). (D) Semi-quantitative graph reporting the percentage of hypusinated eIF5A (hypusinated eIF5A/total eIF5A *100) under the conditions indicated in the figure. Bars represent mean ± standard deviation (n = 3) of the percentage of hypusinated eIF5A.</p> <p>(DOCX)</p>