Adult-specific <i>cyc</i> knockdown does not affect sLNv neuronal morphology.

<p>(A) Representative timeline of the experiments in the figure. Flies were raised in LD at 18°C, and transferred to 28°C immediately after eclosion. Dissections were then performed within days 6–8 post-eclosion at ZT2-3. (B) Representative confocal images of anti-PDF (green) and anti-RFP (magenta) staining in the sLN<sub>v</sub>s when <i>cyc</i> was downregulated exclusively after eclosion using a <i>Pdf-Gal4;tub-Gal80</i><sup><i>ts</i></sup> driver. The images are representative of two independent experiments. Scale bar = 50 μm. Each line also included a <i>Pdf-RFP</i> transgene. Kruskal-Wallis tests followed by Dunn’s multiple comparisons tests were used to quantify the projection length until BP (C), the total number of intersections of the sLN<sub>v</sub> ventral projections (D), the full sLN<sub>v</sub> projection length (E), and the total number of lLN<sub>v</sub> intersections (F). * p < 0.05. Datasets are nonparametric (C-F). Each dot corresponds to one brain. For each genotype: 17 ≤ n ≤ 24. (G-H) Behavioral phenotypes of adult-specific <i>cyc</i> knockdown. Flies were raised in LD at 18°C, before being transferred to 28°C upon eclosion. Experiments were conducted at 28°C. (G) Population Activity plots for flies during days 3–5 of the LD cycle at 18°C (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011441#pgen.1011441.t001" target="_blank">Table 1</a> for additional quantifications). (H) Percent rhythmicity for the indicated genotypes under DD. Fisher’s exact contingency tests were used to analyze the percentage of rhythmic flies under DD (DD1-8). *** p < 0.001. Error bars indicate SEM. For each genotype: 25 ≤ n ≤ 31.</p> <p>(PDF)</p>