<i>T. gondii</i> infection in the jejunum of mice fed brain containing cysts at 3 days post-feeding.

(A) Representative epifluorescent images of jejunum showing SAG1-positive parasites in the stroma and close to the muscular layer. Bottom insets in (A) show a possible immune cell infected by <i>T. gondii</i>. (B) Representative confocal images of jejunum showing parasites replicating in the stroma. Parasites were stained using polyclonal antibodies against <i>T. gondii</i>. (C) Representative confocal 3D projection of jejunum showing SAG-1 positive parasites replicating in the stroma. Intestines are stained for WGA (green), SAG1 (red), polyclonal antibodies against <i>T. gondii</i> (green), and nuclei (blue, DAPI). (PDF)