RVFV detection in whole blood samples from day 3 pi.

<p>Groups of n = 8 A129 mice 8–12 weeks old were inoculated by the IN route with a low (L) or high (H) dose (50 or 1000 pfu, respectively) of the indicated viruses: 40Fp8 (S1A Table), rG1 (S1B Table) and rMP-12 (S1C Table). Each group included 4 Female (F) and 4 Male (M). Whole blood samples collected at day 3 pi were processed to test for viral load by RT-qPCR. Samples rendering a Cq value over o close to 33.0 were further assayed for infectious virus isolation (VI) by inoculation of 5–20 microliters (depending on the availability) on cell culture. A few samples were included as internal controls of the assay (M_L_3; F_L_4). Labelling of the animals is the same as in Figs <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0012011#pntd.0012011.g001" target="_blank">1C</a> and <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0012011#pntd.0012011.g002" target="_blank">2A</a>. Day of death for each animal is indicated. FD: found dead (in some cases blood sample could not be collected); EU: euthanized; S:survivor at day 15 pi (end of the experiment). For RT-qPCR results, the Cq given by the sample is indicated. The results of virus isolation are expressed as follows: NEG (negative, no cpe after 3 passages); (++) (very positive, cpe registered after 1st passage). The specificity of the cpe was confirmed by IFI. ND = not done.</p> <p>(DOCX)</p>