Inc truncations and point mutants lack dominant negative activity in vivo.

Total daily sleep for animals expressing tagged Inc or Inc point mutants under the control of <i>elav</i>^<i>c155</i><i>-Gal4</i> <b>(A-C)</b> or <i>inc-Gal4</i> <b>(D)</b>. <b>(A)</b> <i>elav</i>^<i>c155</i><i>-Gal4</i> expression of 3×FLAG-tagged Inc point mutants. n = 5–24. <b>(B)</b> <i>elav</i>^<i>c155</i><i>-Gal4</i> expression of 3×FLAG-tagged Inc truncations. n = 23–54. <b>(C)</b> <i>elav</i>^<i>c155</i><i>-Gal4</i> expression of Myc-tagged Inc point mutants. Note that Inc^R135H is statistically indistinguishable from Inc and Inc^AAA. n = 18–32. <b>(D)</b> <i>inc-Gal4</i> expression of 3×FLAG-tagged Myc-tagged Inc point mutants. n = 19–63; For all panels, mean ± SEM is shown. Kruskal-Wallis and Dunn’s tests; *p < 0.01; ns, not significant (p > 0.05<b>)</b>. For all panels, animals are heterozygous for UAS transgenes. (PDF)