Co-localisation of TFAM and SYBR Gold staining in HEK-T cells.

Live HEK-T cells; LSM780 confocal microscope, 63x/1.4 Oil objective; 3D image stacks were acquired and used for co-localization analysis. Single optical slice is shown; scale bar 10 μm. Signals in Mitotracker Deep Red (white), TFAM-mEos2 (red) and SYBR Gold (green) channels were acquired sequentially, with switching channels every scanned line. 18 fields of view from two independent transfection experiments were acquired. A representative field of view is shown. Red dashed squares on the left panels mark the region of interest which is shown at higher magnification in the right panels. (PDF)