Phylogenetic analysis of the relatedness of enolase proteins within the class Trematoda.

<p>(A) Maximum likelihood phylogram (unrooted) generated by PhyML based on the protein sequence Met<sup>1</sup>-Arg<sup>429</sup> (Fh_eno_1 nomenclature), from representative enolase sequences from 14 trematode species compared with host (human and sheep) sequences: Cs, <i>Clonorchis sinensis</i>—Cs_eno_1: CSKR_100503, Cs_eno_2: CSKR_104864; Dd, <i>Dicrocoelium dendriticum</i>—Dd_eno_1: DDND1_54290; Ec, <i>Echinostoma caproni</i>—Ec_eno_1: ECPE_0000965901; Fh, <i>Fasciola hepatica</i>—Fh_eno_1: FhHiC23_g15231, Fh_eno_2: FhHiC23_g9518; Fg, <i>Fasciola gigantica</i>—Fg_eno_1: GWHPAZTT000715, Fg_eno_2: GWHPAZTT010334; Fb, <i>Fasciolopsis buski</i>—Fb_eno_1: FBUS_03870, Fb_eno_2: FBUS_04083; Ha, <i>Heterobilharzia americana</i>—Ha_eno_1: HAMR1_26230; Hs, <i>Homo sapiens</i>—Hs_alpha: NP_001419, Hs_beta: NP_001361452, Hs_gamma: NP_001966; Oa, <i>Ovis aries</i>—Oa_alpha: XP_042113226, Oa_beta: A0A6P3TWQ3, Oa_gamma: W5P5C0; Ov, <i>Opisthorchis viverrini</i>—Ov_eno_1: T265_10327, Ov_eno_2: T265_03262; Ph, <i>Paragonimus heterotremus</i>—Ph_eno_1: PHET_04041, Ph_eno_2: PHET_02710, Ph_eno_3: PHET_06263; Sb, <i>Schistosoma bovis</i>—Sb_eno_1: ACC78611; Sh, <i>Schistosoma haematobium</i>—Sh_eno_1: MS3_00003995; Sj, <i>Schistosoma japonicum</i>—Sj_eno_1: ACV41761; Sm, <i>Schistosoma mansoni</i>—Sm_eno_1: Smp_024110; Tr, <i>Trichobilharzia regenti</i>—Tr_eno_1: TREG1_20000. Bootstrap support values (1000 iterations) are shown at each node. (B) Clustal Omega alignment of the enolase sequences from the class Trematoda, compared to sequences from human and sheep as representative host species for <i>Fasciola hepatica</i>. Accession number/protein identifiers used as above.</p> <p>(DOCX)</p>