The increased expression of one or more advantageous genes modestly improves growth.

(A) Comparison of the Bub3-aa strains with control plasmids (pEmpty or p<i>BUB3</i>) and single or double <i>CEN</i> plasmids. Saturated yeast cultures were serially diluted, spotted on YPD plates with and without Rapamycin to a final concentration of 1μg/mL, and imaged after 40 hours of incubation. The comparison of doubling times (by measuring the non-linear regression in the change of OD<sub>600</sub> from 0.5 to 1). The cells were grown to saturation, diluted to 0.1 OD<sub>600</sub> and the OD<sub>600</sub> was measured for 20 hours. (PDF)

Publication Details

Journal
Figshare
Published
2025-02-04
DOI
https://doi.org/10.1371/journal.pgen.1011576.s005
Primary Topic
Education Methods and Technologies
Type
article
Field-Weighted Citation Impact
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The increased expression of one or more advantageous genes modestly improves growth.

Figshare
Education Methods and Technologies
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The increased expression of one or more advantageous genes modestly improves growth.

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Abstract

(A) Comparison of the Bub3-aa strains with control plasmids (pEmpty or p<i>BUB3</i>) and single or double <i>CEN</i> plasmids. Saturated yeast cultures were serially diluted, spotted on YPD plates with and without Rapamycin to a final concentration of 1μg/mL, and imaged after 40 hours of incubation. The comparison of doubling times (by measuring the non-linear regression in the change of OD<sub>600</sub> from 0.5 to 1). The cells were grown to saturation, diluted to 0.1 OD<sub>600</sub> and the OD<sub>600</sub> was measured for 20 hours. (PDF)

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The increased expression of one or more advantageous genes modestly improves growth. · Figshare (2025) | TGRS Research Map | TGRS