Different PIPs-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract.

<p>PIPs and control empty beads were incubated in nuclear lysates for 1 h at 4°C, washed, and subjected to WB detection of BRD4 protein. WB signals for each pull-down condition in each replicate were normalized to the highest signal (PI(4,5)P2). Statistical analysis was performed by Student’s t-test (n = 4). Error bars correspond to SEM (** P < 0.001, **** P < 0.0001).</p> <p>(PDF)</p>